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H-019-29
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Urocortin III (Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-019-29
100 µl
$450.00
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Rat, Mouse brain tissue and human pancreas tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Urocortin III (Mouse) Antiserum (Catalog No.:H-019-29)
Optimal Dilution
1:500 (overnight at 4 °C)
Secondary Antibody
Goat anti-Rabbit IgG, HRP conjugated
Substrate
DAB (Sigma), 10"
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-019-29
Standard Size
100 µl
Sequence
Phe - Thr - Leu - Ser - Leu - Asp - Val - Pro - Thr - Asn - Ile - Met - Asn - Ile - Leu - Phe - Asn - Ile - Asp - Lys - Ala - Lys - Asn - Leu - Arg - Ala - Lys - Ala - Ala - Ala - Asn - Ala - Gln - Leu - Met - Ala - Gln - Ile - NH2
Species
Mouse
Host
Rabbit
Reconstitution
For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 100µl of Rabbit Anti-Urocortin III (Mouse) Serum in the lyophilized form.
Recommended Dilution Factor
Direct ELISA: 1: 3200
Immunohistochemistry: 1:500
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