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H-005-19
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LANP / ANP (26-55), prepro (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-005-19
50 µl
$175.00
ANP (Atrial Natriuretic Peptide)
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Rat and Mouse heart tissues
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
N/A
Blocking
3% H
2
O
2
, 2% Normal Goat Serum
Primary Antibody
Rabbit anti-ANP, Prepro (26-55) (H) antibody (Cat. No.: H-005-19)
Optimal Dilution
1:500
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (sigma), 30min
Counterstained
Hematoxylin, 30Sec
Catalog #
H-005-19
Family
LANP
Standard Size
50 µl
Sequence
Asn - Pro - Met - Tyr - Asn - Ala - Val - Ser - Asn - Ala - Asp - Leu - Met - Asp - Phe - Lys - Asn - Leu - Leu - Asp - His - Leu - Glu - Glu - Lys - Met - Pro - Leu - Glu - Asp -
Species
Human
Host
Rabbit
Reconstitution
For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of rabbit anti- prepro-ANF (26-55) (Human) serum in the lyophilized form.
Recommended Dilution Factor
Immunofluorescence: 1:200
ABC: 1:500
Cross Reactivity
Peptide
% Crossreactivity
LANP/ANP (26-55), prepro (Human)
100
Alpha-ANP (1-28) (Human, Canine, Porcine, Bovine)
0
ANP (Rat)
0
Beta-ANP (Human)
0
Prepro-ANP (56-92) (Human)
0
BNP-32 (Human)
0
CNP-22 (Human, Porcine, Rat)
0
Insulin
0
IGF-1
0
Alphabet_A_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this peptide:
Lundberg et al. Natriuretic peptide expression in the heart of the TTR-ANP transgenic mouse-Comparison to the normal heart.
Microsc Res Tech. 2005 Oct;68(2):97-106.
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