Endorphin, beta (Rat) - Antibody for Immunohistochemistry

多肽

标记多肽

多肽激素文库

抗体

免疫组化抗体
纯化免疫球蛋白

抗体标记

免疫试剂盒

生物标志物阵列

Endorphin, beta (Rat) - Antibody for Immunohistochemistry

Catalog#	Standard size	Price	Order
H-022-33	50 µl	$175.00

Image

Procotol for Immunohistochemistry
Tissue Sample	Rat Cortex
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Endorphin, beta (Rat) AntiSerum (Catalog No.: H-022-33)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-022-33

Standard Size

50 µl

Sequence

Tyr - Gly - Gly - Phe - Met - Thr - Ser - Glu - Lys - Ser - Gln - Thr - Pro - Leu - Val - Thr - Leu - Phe - Lys - Asn - Ala - Ile - Ile - Lys - Asn - Val - His - Lys - Lys - Gly - Gln

Species

Rat

Host

Rabbit

Immunogen

Beta Endorphin (Rat)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-b-Endorphin Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence - 1:200
PAP or ABC - 1:500
Immunocytochemistry - 1:200 (J Endod. 2007 Nov;33(11):1319-24.)

Cross Reactivity

*Peptide*	*% Crossreactivity*
Beta-Endorphin (Rat)	100
Beta-Endorphin (Porcine)	100
Beta-Endorphin (Camel, Ovine, Bovine)	100
Beta-Endorphin (Equine)	100
Alpha-Endorphin	100
Gamma-Endorphin (Human)	60
Beta-Endorphin (Human)	40
Met-Enkephalin	0
Leu-Enkephalin	0

Alphabet_E_

Please click here or click the reference tab on the top to see more articles that use this product.