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Cathepsin S (293-331) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-82B 100 l $450.00

Image

   

   

Protocol for Immunohistochemistry:

 Tissue Sample

 Mouse & rat visceral fat tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 Pre-immuno serum

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Cathepsin S (298-331) (Human) Serum (Catalog No.:H-002-82)

 Optimal Dilution

 1:500 (1hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-82B
Standard Size100 l
SequencePlease call for status of this Item
SpeciesHuman
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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