Gastrin Releasing Peptide (GRP) (Porcine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human Stomach/Colon Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-GRP (Porcine) AntiSerum (Catalog No.: H-027-13)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-027-13

Family

Gastrin Relasing Peptide

Standard Size

50 µl

Sequence

Ala - Pro - Val - Ser - Val - Gly - Gly - Gly - Thr - Val - Leu - Ala - Lys - Met - Tyr - Pro - Arg - Gly - Asn - His - Trp - Ala - Val - Gly - His - Leu - Met - NH2

Species

Porcine

Host

Rabbit

Immunogen

Gastrin Releasing Peptide (Porcine)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit Anti-Gastrin Releasing Peptide (Porcine) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Cross Reactivity

*Peptide*	*%Cross-reactivity*
Gastrin Releasing Peptide (Porcine)	100
Gastrin Releasing Peptide (Human)	100
Big Gastrin I	0
Gastrin I	0
Bombesin	0
Insulin (Human)	0
Substance P	0
Amylin Amide (Human)	0

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