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Cannabinoid Receptor CB2 (323-360) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-81 100 l $250.00

Image

    

Protocol for Immunohistochemistry:

 Tissue Sample

 Mouse & rat brain tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 Pre-immuno serum

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Cannabinoid Receptor CB2 (323-360) (Human) Serum (Catalog No.:H-002-81)

 Optimal Dilution

 1:250~500 (1hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-81
Standard Size100 l
SequenceGly - Leu - Gly - Ser - Glu - Ala - Lys - Glu - Glu - Ala - Pro - Arg - Ser - Ser - Val - Thr - Glu - Thr - Glu - Ala - Asp - Gly - Lys - Ile - Thr - Pro - Trp - Pro - Asp - Ser - Arg - Asp - Leu - Asp - Leu - Ser - Asp - Cys
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100l of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100μl NET Rabbit Antiserum in Lyophilized powder without NaN3.
Recommended Dilution FactorIndirect ELISA      1: 6400
Immunohistochemistry      1: 250~ 500
Western blot: 1: 1000
Cross ReactivityIt is cross reactivity with human CB2 (323-360). It was tested in immunohistochemistry in mouse and rat brain tissues. Not yet tested in other species.
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