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Vaspin (386-414) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-66 100 l $450.00

Image

Western Blot Analysis of Vaspin in rat visceral white adipose tissue

 Tissue Sample Rat visceral white adipose tissue homogenate
 Electrophoresis 10% SDS-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-Vaspin (386-414) (Human) Antiserum (Catalog No.:H-002-66)
 Optimal Dilution1:100-~200 (4 hour at RT, or overnight at 4' C)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate

    

Protocol for Immunohistochemistry:

 Tissue Sample

 Mouse & rat visceral white fat tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 Pre-immuno serum

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Vaspin (386-414) (Human) Serum (Catalog No.:H-002-66)

 Optimal Dilution

 1:500 (1hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-66
Standard Size100 l
SequenceIle - Asp - Lys - Pro - Tyr - Leu - Leu - Leu - Ile - Tyr - Ser - Glu - Lys - Ile - Pro - Ser - Val - Leu - Phe - Leu - Gly - Lys - Ile - Val - Asn - Pro - Ile - Gly - Lys
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100l of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti- Vaspin (386-414) (Human) serum in the lyophilized form. The HPLC purified standard peptide, Vaspin (386-414) (Human) can be detected with this antiserum (1:32000) in ELISA.
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