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Chemerin / TIG2 (135-154) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-51 100 µl $495.00

Image     

Protocol for Immunohistochemistry:

 Tissue Sample

 Human Hepatoma tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 N/A

 Blocking

 3% H2O2, 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-TIG2 /Chemerin (135-154) (H) Antiserum (Catalog No.: H-002-51)

 Optimal Dilution

 1:500 (1 hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-51
Standard Size100 µl
SequenceCys - Leu - Arg - Val - Gln - Arg - Ala - Gly - Glu - Asp - Pro - His - Ser - Phe - Tyr - Phe - Pro - Gly - Gln - Phe
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti-TIG-2 (135-154) serum in the lyophilized form.
Recommended Dilution FactorImmunohistochemistry - 1:500
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