Protocol for Immunohistochemistry:
Tissue Sample
Human Hepatoma tissue
Fixative
10% formalin
Embedding
Paraffin
Negative Control
No primary antibody
Pretreatment
N/A
Blocking
3% H2O2, 2% Normal Goat Serum
Primary Antibody
Rabbit Anti-TIG2 /Chemerin (135-154) (H) Antiserum (Catalog No.: H-002-51)
Optimal Dilution
1:500 (1 hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec