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Bombesin Receptor Subtype-3 (BRS-3) (371-399) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-82 100 l $450.00

Image

Protocol for Immunohistochemistry: 
 Tissue Sample Rat stomach tissue  
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Bombesin Receptor Subtype-3 (371-399) Serum (Catalog No.:H-001-82)
 Optimal Dilution 1:50 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-001-82
Standard Size100 l
SequenceVal - Pro - Gly - Thr - Gly - Ser - Ile - Gln - Met - Ser - Glu - Ile - Ser - Val - Thr - Ser - Phe - Thr - Gly - Cys - Ser - Val - Lys - Gln - Ala - Glu - Asp - Arg - Phe
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti- Receptor Subtype-3 (BRS-3) (371-399) (Human) serum in the lyophilized form
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