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QRFP receptor AQ27 / SP9155 (401-433) (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-81 100 l $450.00

Image

Western Blot Analysis of GPR103 /AQ27 in rat brain tissue
 Tissue Sample Rat brain tissue homogenate
 Electrophoresis 10% SDS-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-GPR103/ AQ27 (401-433) (Human) Antiserum (Catalog No.:H-001-81)
 Optimal Dilution 1:500 (4 hour at RT, or overnight at 4' C)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate

     

Protocol for Immunohistochemistry:
 Tissue Sample Rat or Mouse brain Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-GPR103/ AQ27 (401-433) (Mouse) Antiserum (Catalog No.:H-001-81)
 Optimal Dilution 1:50~100 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification
 ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec
Catalog #H-001-81
Standard Size100 l
SequenceCys - Glu - Gln - Pro - Gly - Glu - Lys - Arg - Gln - Leu - Lys - Arg - Gln - Leu - Ala - Phe - Phe - Ser - Ser - Glu - Leu - Ser - Glu - Asn - Ser - Thr - Phe - Gly - Ser - Gly - His - Glu - Leu
SpeciesMouse
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti- AQ 27 (401-433) (Mouse) serum in the lyophilized form.
Recommended Dilution Factor1: 500 (western blot)
1: 6400 (indirect ELISA)
1: 100 (Immunohistochemistry-ABC) 
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