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Adiponectin Precursor [Cys0] (223-244) (Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-54A 100 l $275.00

Image

 

Protocol for Western Blot: 
 Sample Rat serum, plasma, visceral fat tissue
 Electrophoresis 10% SDS-PAGE gel, reducing condition
 Denatured sample 100'C for 5 min
 Positive control
 Adiponectin (223-244), Cys0, (Rat) peptide
 Blocking
 1% BSA in TBST
 Primary Antibody Rabbit Anti-Adiponectin (222-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
 Optimal Dilution 1:500 (overnight at cold room)
 Secondary Antibody
 Rabbit anti-Mouse IgG, HRP conjugated (1: 12500)
 Substrate ECL
 Positive Band
31 ~32 KDa

 

Protocol for Immunohistochemistry: 
 Tissue Sample Rat visceral white fat tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary AntibodyRabbit Anti-Adiponectin (223-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
 Optimal Dilution 1:500~1000 (overnight hour at 4 0C)
 Secondary AntibodyGoat anti-Rabbit IgG, HRP conjugated
 Amplification  ABC (Vector)
 Detection system HRP
 Substrate DAB (Sigma)
 Counterstained Hematoxylin
Catalog #H-028-54A
Standard Size100 l
SpeciesRat
HostRabbit
ReconstitutionReconstitute with 100 ul or 200 ul distill water or PBS
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor1: 3200 ( indirect ELISA )
1: 500 ~ 1000 (Western blot analysis)
1: 500 ~ 1000 (Immunohistochemistry)
Cross ReactivityThis antibody is crossreactivity with rat adiponectin (223-244). It also tested in rat white fat tissue in western blot analysis and immunohistmochemistry.
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