Rat hypothalamus was stained by Anti-Apelin-36 (Human) Serum (H-057-15)Brailoiu et al. Neurosci Lett. 2002 Jul 26;327(3):193-7.
Immunocytochemistry MethodsCryostat cut tissue sections (10 Am for atrial and ventricular myocardium, lung, kidney and adrenal gland. 30 Am for saphenous vein, radial artery, left internal mammary artery and coronary artery) were left to dry overnight at room temperature. Tissue was fixed in ice-cold acetone for 10 min. Sections were incubated with 5% non-immunized swine serum in PBS for 1 h at room temperature to block nonspecific protein interaction. Tissues were stained using rabbit-anti-apelin-36 (human) antiserum at a dilution of 1:200 in PBS/T for 24 h at 4oC. Tissue sections were then washed three times for 5 min in cold PBS/T before the incubation with swine-anti-rabbit antiserum at a dilution of 1:200 in 1%SS PBS/T for 1 h at room temperature. After repeated washing, sections were incubated with rabbit peroxidase/anti-peroxidase complex at a 1:400 dilution in 1%SS PBS/T. Further washing preceded a 3-min incubation of tissue sections with a 2,5% solution of 3,3V-diaminobenzidine in 0.05M Tris-HCl buffer containing 0.3% hydrogen peroxide. The chromogenic reaction was stopped by immersing the slides in distilled water. Sections were dehydrated using a graded alcohol series before being submerged in xylene for 1 h to clear. Sections were mounted using DePeXGurr mounting medium and then examined using a standard light field microscope (Reichert-Jung, Austria). Kleinz et al. Regul Pept. 2004 May 15;118(3):119-25.
Fig. 5. Apelin-like immunoreactivity in vascular endothelial cells of large conduit vessels. Photomicrograph shows staining in saphenous vein.
Fig. 3. Photomicrograph demonstrating apelin-like immunoreactivity (apelin-LI) in the human heart. Apelin-LI is expressed in vascular endothelial cells of right atria.
Kleinz et al. Regul Pept. 2004 May 15;118(3):119-25.
Links to publications that use this antibody:
Pluvinet et al. CD40: an upstream master switch for endothelial cell activation uncovered by RNAi-coupled transcriptional profiling.Blood. 2008 Nov 1;112(9):3624-37.
Wang et al. Immunohistochemical localization of apelin in human normal breast and breast carcinoma.J Mol Histol. 2008 Feb;39(1):121-4.Han et al. Increased colonic apelin production in rodents with experimental colitis and in humans with IBD.Regul Pept. 2007 Aug 16;142(3):131-7.Atluri et al. Ischemic heart failure enhances endogenous myocardial apelin and APJ receptor expression. Cell Mol Biol Lett. 2007;12(1):127-38.
Xie et al. Apelin and its receptor are expressed in human osteoblasts. Regul Pept. 2006 May 15;134(2-3):118-25.
Susaki et al. Apelin cells in the rat stomach.Regul Pept. 2005 Jul 15;129(1-3):37-41.
Kleinz et al. Immunocytochemical localization of the endogenous vasoactive peptide apelin to human vascular and endocardial endothelial cells.Regul Pept. 2004 May 15;118(3):119-25.
Wang et al. Apelin, a new enteric peptide: localization in the gastrointestinal tract, ontogeny, and stimulation of gastric cell proliferation and of cholecystokinin secretion.Endocrinology. 2004 Mar;145(3):1342-8.