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H-007-65
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VGF, prepro (23-62) (Human, Rat) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-007-65
100 µl
$450.00
Chromogranin B
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Human Pancreas
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-VGF, prepro (23-62) (Human, Rat) Serum (Catalog No.:H-007-65)
Optimal Dilution
1:800 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-007-65
Family
VGF
Standard Size
100 µl
Sequence
Ala - Pro - Pro - Gly - Arg - Pro - Glu - Ala - Gln - Pro - Pro - Pro - Leu - Ser - Ser - Glu - His - Lys - Glu - Pro - Val - Ala - Gly - Asp - Ala - Val - Pro - Gly - Pro - Lys - Asp - Gly - Ser - Ala - Pro - Glu - Val - Arg - Gly - Ala
Species
Human, Rat
Host
Rabbit
Immunogen
VGF, prepro (23-62) (Human, Rat)
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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