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VGF, prepro (23-62) (Human, Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-007-65 100 µl $450.00

Image

Protocol for Immunohistochemistry

Tissue SampleHuman Pancreas
Fixative10% Formalin
EmbeddingParaffin
Negative controlNo primary antibody
PretreatmentTarget retrieval 25 min (Steam)
Blocking2% Normal Goat Serum
Primary AntibodyRabbit Anti-VGF, prepro (23-62) (Human, Rat) Serum  (Catalog No.:H-007-65)
Optimal Dilution1:800 (1hour at RT)
Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection systemHRP
SubstrateDAB (Sigma), 3 min
CounterstainedHematoxylin, 30 Sec
Catalog #H-007-65
FamilyVGF
Standard Size100 µl
SequenceAla - Pro - Pro - Gly - Arg - Pro - Glu - Ala - Gln - Pro - Pro - Pro - Leu - Ser - Ser - Glu - His - Lys - Glu - Pro - Val - Ala - Gly - Asp - Ala - Val - Pro - Gly - Pro - Lys - Asp - Gly - Ser - Ala - Pro - Glu - Val - Arg - Gly - Ala
SpeciesHuman, Rat
HostRabbit
ImmunogenVGF, prepro (23-62) (Human, Rat)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_V_

%007-65%

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