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NERP-4 /Prepro-VGF (485-503) (Human, Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-007-71 100 ul $450.00

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 Protocol for Immunohistochemistry: 

 Tissue SampleHuman Pancreas Tissue
 Fixative10% Formalin
 EmbeddingParaffin
 Negative controlNo primary antibody (pre-immuno serum)
 PretreatmentIntact
 Blocking2% Normal Goat Serum
 Primary AntibodyRabbit Anti-NERP-4/Prepro-VGF (485-503) (Human, Rat, Mouse)  Antiserum (Catalog No.:H-007-71)
 Optimal Dilution1:800 (1hour at RT)
 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 AmplificationABC (Vector) (1:400, 30 min)
 Detection SystemHRP
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 Sec

Catalog #H-007-71
FamilyVGF
Standard Size100 ul
SpeciesHuman, Rat, Mouse
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti-NERP-4 (Human, Rat, Mouse) Serum in the lyophilized form.
Alphabet_N_

%007-71%

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