CART, Research Abstracts, Photomicrographs & Sequences

CART Preprohormone Schematic (Rat)

Prepro-CART Sequence (Rat)

Catalog #	003-62
Standard Size	100 µg
Sequence	Ile - Pro - Ile - Tyr - Glu - Lys - Lys - Tyr - Gly - Gln - Val - Pro - Met - Cys - Asp - Ala - Gly - Glu - Gln - Cys - Ala - Val - Arg - Lys - Gly - Ala - Arg - Ile - Gly - Lys - Leu - Cys - Asp - Cys - Pro - Arg - Gly - Thr - Ser - Cys - Asn - Ser - Phe - Leu - Leu - Lys - Cys - Leu Disulfide Bridge: Cys10 - Cys40Cys14 - Cys32 and Cys34 - Cys47
Species	Rat, Mouse, Bovine
M.W	5259.26
Mass Spec Analysis	Exhibits correct M.W.
Solubility	Before reconstitution, please allow 25 to 45 minutes for the lyophilized peptide to return to room temperature. Briefly vortex the Eppendorf tube to concentrate all material at the bottom of the tube. Gently open the cap and reconstitute the peptide to the desired concentration by adding a sterile, aqueous solution to the tube. Invert the tube 5 times by hand to ensure a homogeneous solution.
Storage Condition	Store peptide in DRY form at 0-5°C.
Appearance	White powder
Content	The contents of this vial have been accurately determined to contain 100µg of NET peptide.
HPLC Analysis	Purity ≥95%
Additional articles	For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this peptide:

Kramer et al. Nicotine administration effects on feeding and cocaine–amphetamine-regulated transcript (CART) expression in the hypothalamus
Regul Pept. 2007 Feb 1;138(2-3):66-73.

Wu et al. CART peptide promotes the survival of hippocampal neurons by upregulating brain-derived neurotrophic factor.
Biochem Biophys Res Commun. 2006 Sep 1;347(3):656-61.

Kappeler et al. Pituitary cocaine- and amphetamine-regulated transcript expression depends on the strain, sex and oestrous cycle in the rat.
J Neuroendocrinol. 2006 Jun;18(6):426-33.

Sliwowska et al. The premammillary hypothalamic area of the ewe: anatomical characterization of a melatonin target area mediating seasonal reproduction.
Biol Reprod. 2004 Jun;70(6):1768-75.

Hwang et al. Central pressor effects of CART peptides in anesthetized rats.
Neuropeptides. 2004 Apr-Jun;38(2-3):69-76.

Wortley et al. Cocaine- and amphetamine-regulated transcript in the arcuate nucleus stimulates lipid metabolism to control body fat accrual on a high-fat diet.
Regul Pept. 2004 Feb 15;117(2):89-99.

Fekete et al. Association of Cocaine- and Amphetamine-Regulated Transcript-Immunoreactive Elements with Thyrotropin-Releasing Hormone-Synthesizing Neurons in the Hypothalamic Paraventricular Nucleus and Its Role in the Regulation of the Hypothalamic-Pituitary-Thyroid Axis during Fasting
J Neurosci. 2000 Dec 15;20(24):9224-34.

Dun et al. Cocaine- and amphetamine-regulated transcript peptide in the rat epididymis: an immunohistochemical and electrophysiological study.
Biol Reprod. 2000 Nov;63(5):1518-24.

Calapai et al. Leptin increases serotonin turnover by inhibition of brain nitric oxide synthesis
J Clin Invest. 1999 Oct;104(7):975-82.

For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.