Novel Paradigm of Metabolic Regulation
38 - 41 kDa
Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
The lyophilized protein is stable for at least 6 months if stored at -80 degree C. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
Lyophilized in sterile filtered solution of PBS with 1% CHAPS
Greater than 85% as determined by SDS-PAGE and HPLC analysis
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Wingless-type MMTV (mouse mammary tumor virus) integration site family member 3a
WNT-3a is a member of the WNT family of signaling proteins that play key roles in embryonic development and the maintenance of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of human WNT-3a is a monomeric protein containing 328 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 38 - 41 kDa on SDS-PAGE under nonreducing conditions. StemRD’s WNT-3a is produced from a human cell line overexpressing human Wnt-3a cDNA in animal-free medium. Purification is done by a proprietary process that is distinct from the published method.
The activity was determined by using a TCF reporter gene assay in cultured human cells. The EC50 ranges from 50 - 200 ng/ml.
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