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Adrenomedullin-5 (Porcine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-010-40 100 l $250.00

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Protocol for Immunohistochemistry
 Tissue Sample Human Placenta Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No Primary Antibody
 PretreatmentN/A
 Blocking2% Normal Goat Serum, 3% H2O2
 Primary Antibody

Rabbit anti-Adrenomedullin-5 (Porcine) Antiserum (Cat. No.: H-010-40)

 Optimal Dilution1:500
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 AmplificationStreptavidin-HRP (Vector), 1:400, 30 min
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 sec
Catalog #H-010-40
FamilyAdrenomedullin
Standard Size100 l
SequenceHis - Gln - Val - Ser - Leu - Lys - Ser - Gly - Arg - Leu - Cys - Ser - Leu - Gly - Thr - Cys - Gln - Thr - His - Arg - Leu - Pro - Glu - Ile - Ile - Tyr - Trp - Leu - Arg - Ser - Ala - Ser - Thr - Lys - Glu - Leu - Ser - Gly - Lys - Ala - Gly - Arg - Lys - Pro - Gln - Asp - Pro - Tyr - Ser - Tyr - NH2 Disulfide bridge between Cys11 & Cys16
SpeciesPorcine
HostRabbit
ImmunogenAdrenomedullin-5 (Porcine)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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