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NERP-2 (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-076-91 100 l $250.00

Image    
Protocol for Immunohistochemistry
 Tissue Sample Rat/Mouse Brain Tissue 
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody (pre-immuno serum)
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-NERP-2 (Human) Antibody (Catalog No.:H-076-91)
 Optimal Dilution 1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-076-91
FamilyNERP
Standard Size100 l
SequencepGlu - Ala - Glu - Ala - Thr - Arg - Gln - Ala - Ala - Ala - Gln - Glu - Glu - Arg - Leu - Ala - Asp - Leu - Ala - Ser - Asp - Leu - Leu - Leu - Gln - Tyr - Leu - Leu - Gln - Gly - Gly - Ala - Arg - Gln - Arg - Gly - Leu - Gly - NH2
SpeciesHuman
HostRabbit
Immunogenhuman NERP-2
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
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