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Nesfatin-3, C-Terminal (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-003-27 100 l $250.00

Image

Protocol for Immunohistochemistry: 
 Tissue Sample Human Brain Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody

 Rabbit anti-Nesfatin-3, C-Terminal (Human) antibody (Cat. No.: H-003-27)

 Optimal Dilution 1:500 
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-003-27
Standard Size100 l
SequenceLeu - Gln - Gln - Gly - Ile - Pro - Pro - Ser - Gly - Pro - Ala - Gly - Glu - Leu - Lys - Phe - Glu - Pro - His - Ile
SpeciesHuman
HostRabbit
ReconstitutionReconstitute with 100 ul distilled water.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorIndirect ELISA:  1:3200
Cross Reactivity
Peptide % cross reactivity
 NUCB 2 (401-420) (Human)

 100

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003-27

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