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GLP-1 (7-37) (Human, Rat, Mouse, Porcine, Bovine, Canine, Ovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-13 50 l $150.00

Image

Rat pancreatic and intestinal sections with an antiserum for human GLP-17–37 (Phoenix Pharmaceuticals, Mountain View, California, USA) at a concentration of 1/500. The second antibody was a biotinylated goat anti-rabbit immunoglobulin, and the signal was detected using the Vectastain ABC kit (Vector Laboratories, Burlingame, California, USA) with DAB as a substrate. The section was counterstained with methyl green. Nie et al. J Clin Invest. 2000 Apr;105(7):955-65.

Protocol for Immunohistochemistry: 
 Tissue Sample Rat Pancreatic and intestinal tissue  
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-GLP-1 (7-37) (Human, Rat, Mouse, Porcine) Serum
(Catalog No.:H-028-13)
 Optimal Dilution 1:500 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained

 with methyl green

Catalog #H-028-13
Standard Size50 l
SequenceHis - Ala - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Val - Ser - Ser - Tyr - Leu - Glu - Gly - Gln - Ala - Ala - Lys - Glu - Phe - Ile - Ala - Trp - Leu - Val - Lys - Gly - Arg - Gly
SpeciesHuman, Rat, Mouse, Porcine, Bovine, Ovine, Canine
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Glucagonl-Like Peptide-1 (7-37) (Human, Rat, Mouse) Serum in the lyophilized form.
Recommended Dilution FactorIndirect ELISA: 1: 3200
Immunohistochemistry: 1~500
Cross Reactivity
Peptide % Crossreactivity 
 GLP-1 (7-37) (Human) 100
 GLP-1 (Human)  100
 GLP-1 (7-36) (Human) 0
 GLP-2 (Human) 0
 Glucagon (Human) 0
 Insulin (Human) 0
 Secretin (Porcine) 0
 GIP (Human) 0
 VIP (Human, Porcine, Rat) 0
 GH-RF (Human) 0
Alphabet_G_
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