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GLP-1 (2-24) (Human, Rat, Mouse, Porcine, Bovine, Canine, Ovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-25 100 l $275.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Human Colon & Colon Cancer Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody Rabbit anti-GLP-1 (2-24) (Human) Antibody (Cat. No.: H-028-25)
 Optimal Dilution 1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  Streptavidin-HRP (Vector), 1:400, 30 min
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-028-25
Standard Size100 l
SpeciesHuman, Rat, Mouse, Porcine, Bovine, Ovine, Canine
HostRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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