Rat hypothalamus tissue
No primary antibody
2% Normal Goat Serum
Rabbit Anti-GRP54 (375-396) (Mouse) Serum (Catalog No.:H-001-64)
1:50-100 (1hour at RT)
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
ABC (Vector) (1:400, 30 min)
DAB (Sigma), 3 min
Links to publications that use this antibody:
Richard et al. KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo by oestradiol and gonadotrophin-releasing hormone.J Neuroendocrinol. 2008 Mar;20(3):381-93.
Castellano et al. Expression of KiSS-1 in rat ovary: putative local regulator of ovulation? Endocrinology. 2006 Oct;147(10):4852-62.