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KiSS-1 (112-121) Amide / Kisspeptin-10 / Metastin (45-54) Amide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-048-56 50 l $450.00

Image


Western Blot Analysis of Kiss-1 / Prepro-metastin in rat brain tissue
 Tissue SampleRat brain tissue homogenate
 Electrophoresis10% SDS-PAGE gel
 Negative controlNo primary antibody
 Blocking1% BSA in TBST or 5% dry milk in TBST
 Primary AntibodyRabbit Anti-Metastin (45-54) (Human) Antiserum (Catalog No.:H-048-56)
 Optimal Dilution1:500-~1000 (4 hour at RT, or overnight at 4oC)
 Secondary AntibodyDonkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection systemHRP
 SubstrateSuperSignal Chemiluminescent Substrate

 

Rat brain and spinal cord tissue was stained by this antibody using the following protocol [Neurosci Lett. 2003 Jan 2;335(3):197-201.]

Protocol for Immunohistochemistry: 
Tissue Sample Rat medulla oblongata and spinal cord
Fixative 4%paraformaldehyde/ 4%picric acid
Embedding Paraffin
Negative control No primary antibody or primary antibody pre-absorbed with the metastin (45-54)-NH2 peptide (10µg/ml) overnight
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Metastin (45-54) (Human) Antibody (Code:H-048-56)
Optimal Dilution 1: 1500
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:150)
Amplification  ABC (Vector)
Detection system HRP
Substrate DAB (Sigma)
Counterstained No

Catalog #H-048-56
Standard Size50 l
SequenceTyr - Asn - Trp - Asn - Ser - Phe - Gly - Leu - Arg - Phe - NH2
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti- Metastin (45-54)-NH2 (Human) Serum in the lyophilized form. The HPLC purified standard peptide, Metastin (45-54)-NH2 (Human) can be detected with this antiserum (1:16000, 0.062µl/ml) in ELISA.
Recommended Dilution FactorImmunohistochemistry - 1:1000. Neurosci Lett. 2003 Jan 2;335(3):197-201.
Immunohistochemistry - 1:1000. Neurosci Lett. 2007 Jun 8;420(1):12-7.
1: 500 ~ 1000 (western blot)
1: 6400 (indirect ELISA)
1: 500~ 1000 (Immunohistochemistry-ABC)
Cross ReactivityThis antibody is crossreactivity with human , rat and mouse KiSS-1 (112-121)-Amide / Kisspeptin-10 / Metastin (45-54)-NH2 peptide at 100%. It has been tested in rat or mouse brain tissue.
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