Parathyroid Hormone and its Related Protein

PTHrP and UCP1

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-056-04
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 57 pg/ml
Range	0-10000 pg/ml
Linear Range	57-1031 pg/ml
Cross Reactivity	Peptide %Cross-reactivity PTH-rP (1-34) (Human) 100 PTH-rP (1-37) (Human) 100 PTH-rP (7-34) Amide (Human) 100 PTH-rP (Chicken) 0 PTH (1-34) (Human) 0 PTH (1-34) (Rat) 0 [Asn76]-PTH 184 (Human) 0 PTH-rP (38-64) Amide (Human) 0 PTH-rP (67-86) Amide (Human) 0 PTH-rP (107-138) (Human) 0 PTH-rP (109-141) (Human) 0
Sequence	Ala - Val - Ser - Glu - His - Gln - Leu - Leu - His - Asp - Lys - Gly - Lys - Ser - Ile - Gln - Asp - Leu - Arg - Arg - Arg - Phe - Phe - Leu - His - His - Leu - Ile - Ala - Glu - Ile - His - Thr - Ala
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	57-1031 pg/ml
ED50	178.87 pg/ml
Sample Level	29.1 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.