CART, Research Abstracts, Photomicrographs & Sequences

Cocaine and Amphetamine-Regulated Transcript (CART) Protein Precursor Schematic (Human)

CART Preprohormone Schematic (Rat)

Prepro-CART Sequence (Rat)

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-003-61
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 85 pg/ml
Range	0-10000 pg/ml
Cross Reactivity	Peptide %Cross-reactivity  CART (61-102) (Human, Rat, Mouse, Bovine)  100  CART (55-102) (Rat, Mouse, Bovine)  9.85  Agouti-Related Protein(83-132)-NH2 (Human)  0  Leptin (Human)  0  MCH (Human)  0  Neuropeptide Y  0  α-MSH  0  Orexin A (Human, Mouse, Rat)  0  Orexin B (Human)  0
Sequence	Lys - Tyr - Gly - Gln - Val - Pro - Met - Cys - Asp - Ala - Gly - Glu - Gln - Cys - Ala - Val - Arg - Lys - Gly - Ala - Arg - Ile - Gly - Lys - Leu - Cys - Asp - Cys - Pro - Arg - Gly - Thr - Ser - Cys - Asn - Ser - Phe - Leu - Leu - Lys - Cys - Leu [Disulfide bridges between Cys8&Cys26,Cys14&Cys34 and Cys28&Cys41]
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	85-732 pg/ml
ED50	195.6 pg/ml
Sample Level	89 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.