Endorphin

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-022-33
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube in 100 µl of Standard Solution = 10~1280 pg/ml
Lowest Detection Limit	27 pg/ml
Cross Reactivity	Peptide %Cross-reactivity  β-Endorphin (Rat)  100  β-Endorphin (Mouse, Porcine)  100  β-Endorphin (Camel, Ovine, Bovine)  100  β-Endorphin (Equine)  100  α-Endorphin  100  β-Endorphin (Human)  40  Met-Enkephalin  0  Leu-Enkephalin  0  α-MSH  0  ACTH (Human)  0  PACAP 38 (Human, Ovine, Rat)  0
Sequence	Tyr - Gly - Gly - Phe - Met - Thr - Ser - Glu - Lys - Ser - Gln - Thr - Pro - Leu - Val - Thr - Leu - Phe - Lys - Asn - Ala - Ile - Ile - Lys - Asn - Val - His - Lys - Lys - Gly - Gln

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Jadwiga Zalewska-Kaszubska, Bartosz Bajer, Dorota Gorska, et al, Effect of repeated treatment with topiramate on voluntary alcohol intake and beta-endorphin plasma level in Warsaw alcohol high-preferring rats. 
Psychopharmacology (Berl). 2013 Jan;225(2):275-81. doi: 10.1007/s00213-012-2812-z. Epub 2012 Jul 31. 

Mizushige T, Saitoh K, Manabe Y, et al. Preference for dietary fat induced by release of beta-endorphin in rats.
Life Sci. 2009;84(21-22):760-5.

Shapiro et al. Synergy between leptin therapy and a seemingly negligible amount of voluntary wheel running prevents progression of dietary obesity in leptin-resistant rats.
Diabetes. 2008 Mar;57(3):614-22.

Zalewska-Kaszubska et al. Voluntary alcohol consumption and plasma beta-endorphin levels in alcohol-preferring rats chronically treated with naltrexone.
Physiol Behav. 2008 Mar 18;93(4-5):1005-10.

Zalewska-Kaszubska et al. Effect of chronic acamprosate treatment on voluntary alcohol intake and beta-endorphin plasma levels in rats selectively bred for high alcohol preference.
Neurosci Lett. 2008 Feb 6;431(3):221-5.

Li et al. Lean rats with hypothalamic pro-opiomelanocortin overexpression exhibit greater diet-induced obesity and impaired central melanocortin responsiveness
Diabetologia. 2007 Jul;50(7):1490-9.

Zalewska-Kaszubska et al. Effect of repeated treatment with topiramate on the beta-endorphin plasma level in rats selectively bred for high and low alcohol preference
Prog Neuropsychopharmacol Biol Psychiatry. 2007 Mar 30;31(2):525-8.

Lutfy et al. Repeated stress alters the ability of nicotine to activate the hypothalamic-pituitary-adrenal axis.
J Neurochem. 2006 Dec;99(5):1321-7.

Zalewska-Kaszubska et al. Effect of acute administration of ethanol on beta-endorphin plasma level in ethanol preferring and non-preferring rats chronically treated with naltrexone.
Pharmacol Biochem Behav. 2006 Sep;85(1):155-9.

Zalewska-Kaszubska et al. Changes in the beta-endorphin plasma level after repeated treatment with acamprosate in rats selectively bred for high and low alcohol preference.
Neurosci Lett. 2005 Nov 4;388(1):45-8.

Piacentini et al. Effect of bupropion on hippocampal neurotransmitters and on peripheral hormonal concentrations in the rat.
J Appl Physiol. 2003 Aug;95(2):652-6.

Miller et al. Obliteration of alpha-melanocyte-stimulating hormone derived from POMC in pituitary and brains of PC2-deficient mice.
J Neurochem. 2003 Aug;86(3):556-63.  

Appleyard et al. A Role for the Endogenous Opioid ß-Endorphin in Energy Homeostasis
Endocrinology. 2003 May;144(5):1753-60.

Olive et al. Stimulation of endorphin neurotransmission in the nucleus accumbens by ethanol, cocaine, and amphetamine.
J Neurosci. 2001 Dec 1;21(23):RC184.

Barrett et al. Does melatonin modulate beta-endorphin, corticosterone, and pain threshold?
Life Sci. 2000;66(6):467-76.

 

tissue extraction: Appelyard SM. - Endocrinology - May 2003; 144: 1753 - 1760 / Piacentini MF. - J Appl Physiol - Apr 2003; 1058 (2002)