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H-010-68
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Salusin-beta (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-010-68
100 µl
$450.00
Salusin
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Rat Brain Tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
N/A
Blocking
3% H
2
O
2
, 2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Salusin-beta (Human) Serum (Catalog No.:H-010-68)
Optimal Dilution
1:200, 1 hour at RT
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-010-68
Standard Size
100 µl
Sequence
Ala - Ile - Phe - Ile - Phe - Ile - Arg - Trp - Leu - Leu - Lys - Leu - Gly - His - His - Gly - Arg - Ala - Pro - Pro - OH
Species
Human
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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