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NEW Periostin ELISA Kit

A secreted protein that associated with obesity, diabetes and cardiovascular diseases

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Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus.

OBJECTIVE:
Skeletal muscle produces a variety of secreted proteins that have important roles in intercellular communication and affects processes such as glucose homoeostasis. The objective of this study was to develop a novel Signal Sequence Trap (SST) in conjunction with cDNA microarray technology to identify proteins secreted from skeletal muscle of Psammomys obesus that were associated with obesity and type 2 diabetes (T2D).

DESIGN:
Secreted proteins that were differentially expressed between lean, normal glucose tolerant (NGT), overweight and impaired glucose tolerant (IGT) and obese, T2D P. obesus were isolated using SST in conjunction with cDNA microarray technology. Subsequent gene expression was measured in tissues from P. obesus by real-time PCR (RT-PCR).

RESULTS:
The SST yielded 1600 positive clones, which were screened for differential expression. A total of 91 (approximately 6%) clones were identified by microarray to be differentially expressed between NGT, IGT and T2D P. obesus. These clones were sequenced to identify 51 genes, of which only 27 were previously known to encode secreted proteins. Three candidate genes not previously associated with obesity or type 2 diabetes, sushi domain containing 2, collagen and calcium-binding EGF domains 1 and periostin, as well as one gene known to be associated, complement component 1, were shown by RT-PCR to be differentially expressed in skeletal muscle of P. obesus. Further characterization of the secreted protein Postn revealed it to be predominantly expressed in adipose tissue, with higher expression in visceral compared with subcutaneous adipose depots.

CONCLUSION:
SST in conjunction with cDNA microarray technology is a powerful tool to identify differentially expressed secreted proteins involved in complex diseases such as obesity and type 2 diabetes. Furthermore, a number of candidate genes were identified, in particular, Periostin, which may have a role in the development of obesity and type 2 diabetes.

Bolton K et al., Int J Obes (Lond). 2009 Oct;33(10):1153-65. doi: 10.1038/ijo.2009.148. Epub 2009 Jul 28.

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Periostin, discovered by nano-flow liquid chromatography and mass spectrometry, is a novel marker of diabetic retinopathy.

Takada M, et al., Biochem Biophys Res Commun., .2010 Aug 20;399(2):221-6. doi: 10.1016/j.bbrc.2010.07.058. Epub 2010 Jul 21.

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Identification of cardiovascular genes in omentum from morbidly obese patients with type 2 diabetes.

Hindle AK, et al., 2010 Jun;34(6):1020-7. doi: 10.1038/ijo.2010.23. Epub 2010 Feb 16.

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High glucose increases periostin expression and the related signal pathway in adult rat cardiac fibroblasts.

Zou P, et al., Acta Physiologica Sinica, 2010 Jun 25;62(3):247-54.

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Levels of blood periostin decrease after acute myocardial infarction and are negatively associated with ventricular function after 3 months.

Cheng CW et al., J Investig Med. 2012 Feb;60(2):523-8. doi: 10.231/JIM.0b013e3182408549.

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The adult heart responds to increased workload with physiologic hypertrophy, cardiac stem cell activation, and new myocyte formation.

Waring CD et al, Eur Heart J. 2012 Oct 25. [Epub ahead of print]

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Metabolic characterization of volume overload heart failure due to aorto-caval fistula in rats.

Melenovsky V et al, Mol Cell Biochem 2011 Aug;354(1-2):83-96. doi: 10.1007/s11010-011-0808-3. Epub 2011 Apr 5.

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Serum periostin levels are correlated with progressive skin sclerosis in patients with systemic sclerosis.

Yamaguchi Y et al., Br J Dermatol. (2012) Oct 30. doi: 10.1111/bjd.12117. [Epub ahead of print]

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Identification of periostin as a critical marker of progression/reversal of hypertensive nephropathy.

Guerrot D, et al., PLoS One. 2012;7(3):e31974. doi: 10.1371/journal.pone.0031974. Epub 2012 Mar 5

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Proteomic Identification of Matrix Metalloproteinase Substrates in the Human Vasculature.

Stegemann C et al., Circ Cardiovasc Genet. 2012 Dec 19. [Epub ahead of print]

Periostin (Human) ELISA Kit (EK-074-41)

Minimum detection level: 0.14 ng/ml
Linear Range: 0.22-54 ng/ml

Periostin Recovery from Human Plasma Samples

    Note: * Human plasma samples (collected with EDTA) were diluted
                with assay buffer at 1:30

** Varying concentrations of periostin were spiked into diluted human plasma which divided into three groups. Data is presented after the un-spiked plasma value is subtracted.

Linearity of test samples for periostin EIA kit (EK-074-41)

   Note: **Human EDTA plasma samples were serially diluted with Dilution
                buffer and assayed

Amino Acid Sequence of human perisotin/ OSF-2

EK-074-41


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