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Synthesis of Irisin and Irisin-derived Peptide-fragments
for its Applications

Presented at “Chinese Peptide Symposium 2012” By Dr. Jaw-Kang Chang

Solid-Phase Synthesis and purification of Irisin

A single peak of purified Irisin was shown in HPLC profile with correct molecular ion (12 charges of M.W. of 1049 Da) in MS spectrometry.

HPLC Profile of Irisin (Purity ≥ 95%)

Western Blot Results

After SDS-PAGE, both the Irisin peptide (12kD Mw, Cat. #067-16) and Irisin (42-112) peptide (8 kD Mw, Cat. #067-17) can be transferred to PVDF membrane and have been detected by Irisin (42-112) (human, Rat, Mouse) IgG (Cat. No. G-067-17) with a diluted concentration at 1: 1500 from the stock (1 µg/µl).

Western Blot Analysis of Irisin immunoreactive bands for human plasma extracted with antibody conjugated magnetic bead

Irisin peptide (12kD Mw, Cat. #067-16), Irisin (42-112) peptide (8 kD Mw, Cat. #067-17) and Irisin precursor (22 kD Mw) can be conjugated with magnetic bead.

 

See more information, please visit Irisin for details.

Irisin

%Irisin%


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