Pair-wise alignment between human and rat INSL6. The two
polypeptides exhibited 47% identity. ":" Denotes a conserved residue.
"." Denotes a conservative substitution. The nucleotide sequences
encoding human and rat INSL6 are available through GenBank accession
nos. AF156094 and AF159506, respectively. The predicted signal peptide
sequences are underlined. The B- and A-domain regions are in shaded
boxes.
Lok S, et al. Biol Reprod. 2000 Jun;62(6):1593-9.
Northern blot analysis of total INSL6 transcript. RNA isolation, fractionation, and hybridization to 32P-labeled INSL6 cDNA are described in Materials and Methods:
a)
Human multiple-tissue mRNA blot (Clontech). Each lane contained
approximately 2 µg of poly(A)+ RNA from the specified tissues.
Hybridization of the blot to a human clathrin probe to confirm the
integrity of the mRNA is shown at the bottom.
b) Rat
mRNA blots. Each lane represents 10 µg of total RNA from the indicated
tissues. The locations of the 28S and 18S ribosomal RNAs are indicated.
Hybridization of the blots to a cyclophilin probe to confirm the
integrity of the mRNA is shown in the lower panels
