Technical Support

	Phoenix Pharmaceuticals, Inc.

If you don't find the answer to your question(s), send us an email for technical support


	Conversion Program of Amino Acid Sequence Codes

FREQUENTLY ASKED QUESTIONS

Click on the topic to find the answers to your FAQ.

	RIA (Radio immunoassay) Kits
	Enzyme Immunoassay (EIA) Kits
	Antibodies
	Tracers
	Storage
	Delivery time
	Peptides
	Plasma Extraction
	What does it mean?
	Blood Samples

RIA (Radio immunoassay) Kits:

	What is the activity of an RIA kit? 1.5 µCi per Kit
	What kind of sample medium can I use? Any biological fluid with a peptide level high enough to be detected by the kit can be used (e.g. plasma, serum, tissue homogenate).
	What is the delivery time of an RIA Kit? We label our tracer every week upon order. Place your order by Monday before noon for delivery of the following Tuesday.
	What is the sample volume used in a RIA Kit? 100µl per tube.
	How many samples can be measured with one RIA Kit? You can measure up to 50 duplicate samples.
	How should the RIA Kit be stored? The kit should be stored at -20°C. We do not recommend storing any rehydrated compounds.
	What is the expiration date of a RIA Kit? The expiration date is approximately 6 weeks after iodination. We strongly recommend that the kit be used as soon as possible for best results.
	What kind of tubes should be used in an RIA Kit? Polypropylene or polystyrene tubes (12 x 75mm) should be used to perform the assay to avoid peptide sticking to the tube walls.
	How to calculate from pg/tube to pmol/l? In short form: The molecular weight can be found at the "Catalog Peptides" section. If you diluted your samples before the assay, please remember to include these in your calculations.

Enzyme Immunoassay (EIA) Kits:

	Is an EIA kit identical with an ELISA Kit? Yes
	What kind of sample medium can be used? Any biological fluid with a peptide level high enough to be detected by the kit can be used (e.g. Plasma, serum, tissue homogenate).
	How should the EIA Kit be stored? The kit should be stored at 2 to 4°C. DO NOT FREEZE.
	How many samples can be measured with one EIA Kit? Up to 40 duplicate samples.
	What is the expiration date for an EIA Kit? The expiration time is approximately 6-12 month after date of shipment.
	What is the sample volume used in an EIA Kit? 50µl per well.
	How often can the EIA Kit be split? The multiwell plate contains 12 strips with 8 wells each.
	What is the delivery time for EIA Kits? Approximately 3 days after receipt of order.

Antibodies:

	What host are the antibodies raised in? All of our antibodies have been raised in rabbits.
	How much peptide has to be injected into the rabbit? 5-10 mg of > 70% pure peptide conjugated to KLH.
	How to store antibodies (before and after rehydration)? Store antibodies at 2-4°C before and at -20°C after rehydration. The product is stable for approximately 3-6 weeks after rehydration at -20°C or below.
	What is the delivery time for antibodies? Approximately 3 days after receipt of order.

Tracer:

	What is the activity of a Tracer? 10 µCi per Tracer.
	How to store tracers (before and after rehydration)? Store at -20 °C before hydration and at 4°C after rehydration. The product is stable for approximately one week after rehydration. We strongly recommend that the kit be used as soon as possible for best results.
	What is the price and delivery time for a custom iodination? Please inquire. info@phoenixpeptide.com

Storage:

	How to store peptides (before and after rehydration)? Store at 2 to 4°C before hydration and at -70°C after rehydration. Most peptides are stable for approximately 3-6 month after rehydration. Typically, any peptide containing Tryptophane, Cystein and Methionine can be easily oxidized and are therefore less stable.
	How should an RIA Kit be stored? The kit should be stored at -20°C.
	How should an EIA Kit be stored? The kit should be stored at 2 to 4°C. DO NOT FREEZE.
	How to store tracers (before and after rehydration)? Store at -20°C before hydration and at 4°C after rehydration. We do not recommend storing any tracer for longer than 2-3 days.
	What is the expiration time for buffers and how to store buffers? Store at 2 to 4°C. The buffers are stable for approximately 6 months.

Delivery time:

	What is the delivery time for peptides? Approximately 3 days after receipt of order.
	What is the delivery time for antibodies? Approximately 3 days after receipt of order.
	What is the delivery time for labeled peptides? Approximately one week after receipt of order.
	What is the delivery time for EIA Kits? Approximately 3 days after receipt of order.
	What is the delivery time of an RIA Kit? We label our tracer every week upon order. Place your order by Monday before noon for delivery on the following Tuesday.
	What is the expiration time for buffers and how to store buffers? Store at 2 to 4°C. The buffers are stable for approximately 6 months.

Peptides:

	What is the price and delivery time for custom synthesized peptides? Approximately US50 per residue. Delivery time is approximately 2 weeks after receipt order.
	What is the delivery time for labeled peptides? Approximately one week after receipt of order.
	How to store peptides (before and after rehydration)? Store at 2 - 4°C before hydration and at -70°C after rehydration. Most peptides are stable for approximately 3-6 month after rehydration. Typically, any peptide containing Tryptophane, Cystein and Methionine can be easily oxidized and are therefore less stable.

Plasma Extraction:

What materials are necessary for plasma extraction?

Code#	Description	Quantity	Price in US
RK-SEPCOL-1	SEP-Columns, 200mg	50 per box	US75
RK-BA-1	Buffer A	500ml	US20
RK-BB-1	Buffer B	100ml	US15

The buffers cover the extraction of 20 samples. The SEP-Columns hold a net volume of 3ml and can NOT be reused.

When is extraction required?
Extraction is strongly recommended but not required. It is up to the discretion of journal and publication reviewers. Extraction is not required for Ghrelin (Human) RIA and EIA Kits, Ghrelin (Rat) RIA and EIA Kits and Resistin (Human) RIA and EIA Kits.

How to convert from rpm (rotations per minute) to g?
Xg=1.11x10^-5xrxrpm²
r=radial distance in cm
rpm=rotational velocity of the motor (rounds per minute)

What does this mean?

	What is the difference between "Antibody for Immunohistochemistry" (H-xxx-xx) and "Purified IgG" (G-xxx-xx)? "Antibody for Immunohistochemistry" is the unpurified serum from the immunized rabbit. The "Purified IgG" is the purified immunoglobulin G from rabbit serum.
	What does (7-26) mean after a peptide's name? This peptide is a fragment of the entire peptide or protein (e.g. fragment from amino acid 7 to amino acid 26 starting at the N-terminal).
	What does (Human, Rat, Mouse) mean after a peptide or kits name? The amino acid sequence for this peptide is identical in Human, Mouse and Rat, the kit can be used for Human, Mouse and Rat samples.
	Where to find references for kits, peptides or antibodies? Available references for our products can be found on our web site.

Blood samples:

	Can EDTA blood be used in the kits? Yes, however for storage of samples we strongly recommend the addition of protease inhibitor, e.g. Aprotinin (RK-APRO).
	At what temperature should the samples be stored? For best results, store the samples at -70°C and avoid freeze thaw.
	How long can the samples be stored? Plasma sample should not be stored for longer than 6-12 months.