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PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
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| Rabbit Anti-AC2-26 (Human)
Antibody for IHC & WB |
| Price: |
450 USD |
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| Catalog No.: |
H-072-13 |
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| Host |
rabbit |
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| Immunogen Sequence: |
human AC2-26 |
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Ac-Ala-Met-Val-Ser-Glu-Phe-Leu-Lys-Gln-Ala-Trp-Phe-Ile-Glu- Asn-Glu-Glu-Gln-Glu-Tyr-Val-Gln-Thr-Val-Lys |
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| Antibody Type: |
Rabbit IgG |
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| Formulation: |
50 ul Lyophilized powder without
NaN3. |
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| Reconstitution: |
Reconstitute with 50 ul distill water or PBS. |
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| Storage: |
Lyophilized antiserum are stable for one year from the data of
receipt when stored at -200C to -700C.Reconstituted antiserum can
also be aliquotted and stored frozen at -200C to -700C in a manual
defrost freezer for six months without detectable loss activity.
Upon reconstitution, the antiserum can be stored at 40C for 1 month.
Avoid repeated freeze-thaw cycles. |
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| Specificity: |
This antibody is crossreactivity with human Ac2-26 at 100%. It was tested in rat tissue in immunohistochmeistry and western blot. |
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| Applications: |
Direct ELISA: 1: 6400 |
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Western blot: 1: 500 ~1000 |
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Immunohistochemistry: 1:500 ~ 1000 |
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Tissue
Sample |
rat heart tissue |
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Fixative |
10% Formalin |
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Embedding |
Paraffin |
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Negative
control |
No primary
antibody |
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Pretreatment |
intact |
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Blocking |
2% Normal
Goat Serum |
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Primary
Antibody |
Rabbit
Anti-AC 2-26 (Human)l Antibody
(Catalog No.:H-072-13) |
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Optimal
Dilution |
1: 500
(overnight at cold room) |
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Secondary
Antibody |
Rabbit anti-Mouse
IgG, HRP conjugated |
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Substrate |
DAB (Sigma),
10" |
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Counterstained |
Hematoxylin,
30 Sec |
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Sample |
rat heart tissue |
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Electrophoresis |
15% Tricine-PAGE gel, reducing condition |
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Denatured sample |
1000C for 5 min |
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Positive
control |
N/A |
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Blocking |
1% BSA in TBST, |
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Primary
Antibody |
Rabbit
Anti-AC2-26 (Human) Monoclonal Antibody
(Catalog No.:H-072-13) |
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Optimal
Dilution |
1:500
(overnight at cold room) |
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Secondary
Antibody |
Rabbit anti-Mouse
IgG, HRP conjugated |
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Substrate |
ECL |
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Positive Band |
~ 35 KDa |
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