INSL4 (Human) Antibody

PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite

Rabbit Anti- INSL5, C Peptide, Prepro (46-109) (Human) Serum for Immunohistochemistry

Catalog No.:	H-035-58

Lot No.:	PBR121-3 or PBR122-3

Immunogen	synthetic Human INSL5, C Peptide, Prepro (46-109)
	His-Leu-Glu-Gly-Ile-Pro-Gln-Ala-Gln-Gln-Ala-Glu-Thr-Gly-Asn-Ser- Phe-Gln-Leu-Pro-His-Lys-Arg-Glu-Phe-Ser-Glu-Glu-Asn-Pro-Ala-Gln- Asn-Leu-Pro-Lys-Val-Asp-Ala-Ser-Gly-Glu-Asp-Arg-Leu-Trp-Gly-Gly- Gln-Met-Pro-Thr-Glu-Glu-Leu-Trp-Lys-Ser

Formulation:	100 ul NET Rabbit serum in Lyophilized powder without NaN3 or any carry protein.

Reconstitution:	Reconstitute with 100 ul distill water or PBS.

Storage:	Lyophilized antiserum are stable for one year from the data of receipt when stored at -20 'C to -70 'C.Reconstituted antiserum can also be aliquotted and stored frozen at -20'C to -70'C in a manual defrost freezer for six months without detectable loss activity. Upon reconstitution, the antiserum can be stored at 4'C for 1 month. Avoid repeated freeze-thaw cycles.

Specificity:	It is cross reactivity with Human INSL5, C peptide, Prepro (46-109). It was tested in immunohistochemistry in rat and mouse brain tissues. Not yet tested in other species.

Applications:	Indirect ELISA: 1: 6400
	Immunohistochemistry:1: 500 ~ 1000

	Tissue Sample	Rat and Mouse Brain tissue
	Fixative	10% Formalin
	Embedding	Paraffin
	Negative control	Pre-immuno serum
	Pretreatment	Intact
	Blocking	2% Normal Goat Serum
	Primary Antibody	Rabbit Anti-INSL5, C Peptide, Prepro (46-109) (Human) Serum (Catalog No.:H-035-58)
	Optimal Dilution	1:250 ~ 500 (Overnight at 4^oC)
	Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
	Amplification	ABC (Vector) (1:400, 30 min)
	Detection system	HRP
	Substrate	DAB (Sigma), 3 min
	Counterstained	Hematoxylin, 30 Sec