|
|
 |
PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
|
|
|
Rabbit Anti- INSL4 (Human) Serum for
human tissue Immunohistochemistry
|
| Catalog No.: |
H-035-33-PB |
| |
|
| Lot No.: |
PBR87-3 or PBR87-4 |
| |
|
| Immunogen |
synthetic Human INSL4 |
| |
 |
| |
|
| Formulation: |
100 ul NET Rabbit serum in Lyophilized powder
without NaN3. |
| |
|
| Reconstitution: |
Reconstitute with 100 ul distill water or PBS. |
| |
|
| Storage: |
Lyophilized antiserum are stable for one year from the data of
receipt when stored at -20 'C to -70 'C.Reconstituted antiserum can
also be aliquotted and stored frozen at -20'C to -70'C in a manual
defrost freezer for six months without detectable loss activity.
Upon reconstitution, the antiserum can be stored at 4'C for 1 month.
Avoid repeated freeze-thaw cycles. |
| |
|
| Specificity: |
It is cross reactivity with Human INSL4. It was tested in immunohistochemistry
in human placenta tissues. Not yet tested in other
species. |
| |
|
| Applications: |
Indirect ELISA: 1: 6400 |
| |
Immunohistochemistry:1: 1000~2000 |
| |
|
 |
|
 |
| |
 |
Tissue
Sample |
human
placenta
tissue |
 |
Fixative |
10% Formalin |
|
Embedding |
Paraffin |
|
Negative
control |
Pre-immuno
serum |
|
Pretreatment |
Intact |
|
Blocking |
2% Normal
Goat Serum |
|
Primary
Antibody |
Rabbit
Anti-INSL4 (Human) Serum (Catalog
No.:H-035-33-PB) |
|
Optimal
Dilution |
1:1000
~ 2000 (Overnight at 4oC) |
|
Secondary
Antibody |
Goat
anti-Rabbit IgG, Biotinylated (1:400, 30 min) |
|
Amplification |
ABC (Vector)
(1:400, 30 min) |
|
Detection
system |
HRP |
|
Substrate |
DAB (Sigma),
3 min |
|
Counterstained |
Hematoxylin,
30 Sec |
|
|
|
 |
|
 |
|
