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Oxyntomodulin (Human, Mouse, Rat) Antibody

Glucagon-37 (Human, Mouse, Rat) Antibody


Catalog No. : H-028-22
   
Lot No:  
   
Immunogen sequence :

His - Ser - Gln - Gly - Thr - Phe - Thr - Ser - Asp - Tyr - Ser - Lys - Tyr - Leu - Asp - Ser - Arg - Arg - Ala - Gln - Asp - Phe - Val - Gln - Trp - Leu - Met - Asn - Thr - Lys - Arg - Asn - Arg - Asn - Asn - Ile - Ala

   
Host Rabbit
   
Formulation 100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.
   
Reconstitution: Reconstitute with 100 ul distill water.
   
Storage: Lyophilized antiserum are stable for one year from the data of receipt when stored at -200C to -70 0C.Reconstituted antiserum can also be aliquotted and stored frozen at -200C to -700C in a manual defrost freezer for six months without detectable loss activity. Upon reconstitution, the antiserum can be stored at 40C for 1 month. Avoid repeated freeze-thaw cycles.
   
Specificity: This antibody is crossreactivity with Oxyntomodulin (Human, Mouse, Rat). It also was tested in immunohistocmheistry in Human and rat stomach tissue. Other tissue does not test.
   
Application:  
  1: 6400 (indirect ELISA)
  1:500 (Immunohistochemistry)
   
Positive control peptide 028-22

 


   
 
   



OXyntomodulin  Immunohistochemistry

 
Tissue Sample Human , Mouse and Rat intestine and pnacreas tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Oxyntomodulin (Human, Mouse, Rat) Antiserum (Catalog No.:H-028-22)
Optimal Dilution 1:500 (overnight at 4oC)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification  ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec