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PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
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Glucagon (19-29) (Human, Mouse, Rat)
Antibody
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| Catalog No. : |
H-028-05 |
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| Lot No: |
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| Immunogen sequence : |
Ala - Gln - Asp - Phe - Val - Gln - Trp - Leu - Met - Asn - Thr |
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| Host |
Rabbit |
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| Formulation |
100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3. |
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| Reconstitution: |
Reconstitute with 100 ul distill water. |
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| Storage: |
Lyophilized antiserum are stable for one year from the data of
receipt when stored at -200C to -70 0C.Reconstituted antiserum can
also be aliquotted and stored frozen at -200C to -700C in a manual
defrost freezer for six months without detectable loss activity.
Upon reconstitution, the antiserum can be stored at 40C for 1 month.
Avoid repeated freeze-thaw cycles. |
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| Specificity: |
This antibody is crossreactivity with Glucagon (19-290 (Human, Mouse, Rat).
It also was tested in immunohistocmheistry
in Human and rat pancreas tissue. Other tissue does not test. |
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| Application: |
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1: 6400 (indirect ELISA) |
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1:500 (Immunohistochemistry) |
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| Positive control peptide |
028-05 |
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Glucagon (19-29) Immunohistochemistry
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Tissue Sample |
Human , Mouse and Rat pnacreas tissue |
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Fixative |
10% Formalin |
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Embedding |
Paraffin |
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Negative control |
No primary antibody |
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Pretreatment |
intact |
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Blocking |
2% Normal Goat
Serum |
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Primary Antibody |
Rabbit Anti-Glucagon (19-29) (Human, Mouse, Rat) Antiserum (Catalog No.:H-028-05) |
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Optimal Dilution |
1:500 (overnight
at 4oC) |
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Secondary Antibody |
Goat anti-Rabbit
IgG, Biotinylated (1:400, 30 min) |
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Amplification |
ABC (Vector) (1:400,
30 min) |
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Detection system |
HRP |
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Substrate |
DAB (Sigma), 3
min |
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Counterstained |
Hematoxylin, 30
Sec |
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