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PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
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| Mouse Anti-Adiponectin (Human)
Monoclonal Antibody |
| Catalog No.: |
MAB-ADI-01-A (100 ug) |
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MAB-ADI-01-B (200 ug) |
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| Clone: |
C11H2 |
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| Host |
Mouse |
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| Immunogen Sequence: |
Human Adiponectin, pET-32A, recombinant
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| Formulation: |
200 ug IgG was purified by protein G affinity in Lyophilized powder
without BSA and NaN3. |
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| Reconstitution: |
Reconstitute with 100 ul distill water and PBS to 200ug/200ul. |
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| Storage: |
Lyophilized Antibody are stable for one year from the data of
receipt when stored at -20 0C to -70 0C.Reconstituted Antibody can
also be aliquotted and stored frozen at -200C to -700C in a manual
defrost freezer for six months without detectable loss activity.
Upon reconstitution, the antibody can be stored at 40C for 1 month.
Avoid repeated freeze-thaw cycles. |
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| Specificity: |
This antibody is crossreactivity with human Adiponectin, recombinant.
It also tested in human plasma samples in western blot analysis and
human visceral white fat tissue in immunohistmochemistry |
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| Positive Band |
single band at 31~ 32 KDa |
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| Applications: |
1: 3200 ( indirect ELISA ) |
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1: 500 ~ 1000 (Western blot analysis) |
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1: 500 ~ 1000 (Immunohistochemistry) |
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| Positive control |
Human Adiponectin, recombinant |
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Immunohistochemistry
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Tissue
Sample |
Human visceral
white fat tissue |
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Fixative |
10% Formalin |
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Embedding |
Paraffin |
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Negative
control |
No primary
antibody |
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Pretreatment |
intact |
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Blocking |
2% Normal
Goat Serum |
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Primary
Antibody |
Rabbit
Anti-Adiponectin (Human) Antibody (Catalog
No.:MAB-ADI-01) |
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Optimal
Dilution |
1:500~1000
(overnight hour at 4 0C) |
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Secondary
Antibody |
Goat
anti-Rabbit IgG, HRP conjugated |
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Substrate |
DAB (Sigma),
10" |
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Counterstained |
Hematoxylin,
30 Sec |
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Wetern Blot Analysis
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Sample |
Human
serum, plasma, rat visceral fat tissue |
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Electrophoresis |
10% SDS-PAGE
gel, reducing condition |
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Denatured
sample |
100'C
for 5 min |
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Positive
control |
recombinant
human Adiponectin, pET-32A |
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Blocking |
1% BSA
in TBST, |
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Primary
Antibody |
Rabbit
Anti-Adiponectin (Human) Monoclonal Antibody (Catalog
No.:MAB-ADI-01) |
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Optimal
Dilution |
1:500
(overnight at cold room) |
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Secondary
Antibody |
Rabbit
anti-Mouse IgG, HRP conjugated (1: 12500) |
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Substrate |
ECL |
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Positive
Band |
31 ~32
KDa |
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