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Urocortin (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-14 50 µl $300.00

Image

Protocol for Immunohistochemistry:
Tissue Sample Rat piriform cortex 
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Pronase  
Blocking 2% Normal Goat Serum 
Primary Antibody Rabbit Anti-Urocortin serum (Catalog No.:H-019-14) 
Optimal Dilution 1:100 (1hour at RT) 
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
Amplification  ABC (Vector) (1:400, 30 min) 
Detection system  HRP
Substrate DAB (Sigma), 3 min 
Counterstained Hematoxylin, 30 Sec 

Catalog #H-019-14
Standard Size50 µl
SequenceAsp - Asn - Pro - Ser - Leu - Ser - Ile - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Thr - Leu - Leu - Glu - Leu - Ala - Arg - Thr - Gln - Ser - Gln - Arg - Glu - Arg - Ala - Glu - Gln - Asn - Arg - Ile - Ile - Phe - Asp - Ser - Val - NH2
SpeciesHuman
HostRabbit
ImmunogenUrocortin (Human)
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Urocortin (Human) Serum in the lyophilized form.
Cross Reactivity
Peptide % Cross-reactivity
Urocortin (Human)  100
Urocortin (Rat)100 
Cst-14 (Rat) 1.94 
CRF (Human, Rat)  
MCH (Human, Mouse, Rat) 
LH-RH (Human, Porcine, Rat) 
Somatostatin  
NPY (Human, Rat) 
Alphabet_U_
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