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Urocortin III (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-29 100 µl $450.00

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Protocol for Immunohistochemistry
Tissue Sample Rat, Mouse brain tissue and human pancreas tissue
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Intact
Blocking 2% Normal Goat Serum 
Primary Antibody Rabbit Anti-Urocortin III (Mouse) Antiserum (Catalog No.:H-019-29)
Optimal Dilution 1:500 (overnight at 4 °C)
Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
Substrate DAB (Sigma), 10"
Counterstained Hematoxylin, 30 Sec

Catalog #H-019-29
Standard Size100 µl
SequencePhe - Thr - Leu - Ser - Leu - Asp - Val - Pro - Thr - Asn - Ile - Met - Asn - Ile - Leu - Phe - Asn - Ile - Asp - Lys - Ala - Lys - Asn - Leu - Arg - Ala - Lys - Ala - Ala - Ala - Asn - Ala - Gln - Leu - Met - Ala - Gln - Ile - NH2
SpeciesMouse
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti-Urocortin III (Mouse) Serum in the lyophilized form.
Recommended Dilution FactorDirect ELISA: 1: 3200
Immunohistochemistry: 1:500
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