VIP (Vasoactive Intestinal Peptide /PHI and Related Peptides)

Prepro-VIP/PHM-27 Schematic (Human)

Normal Plasma Peptide/Protein Levels

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-064-16
Standard Size	96 well plate
Sensitivity	Minimum Detectable Concentration = 13.3 pg/mL
Range	0-10000 pg/ml
Linear Range	13.3-301 pg/ml
Cross Reactivity	Peptide Cross-reactivity VIP (Human, Porcine, Rat) 100 VIP (Guinea Pig) 100 VIP (10-28) (Human, Porcine, Rat) 34.6 VIP (Chicken) 28 PACAP-27-NH2 (Human, Rat, Ovine)          <0.02 VIP (1-12) (Human, Porcine, Rat) 0 PHM-27 (Human) 0 Substance P 0 Endothelin-1 (Human, Rat, Porcine,Mouse, Canine, Bovine) 0 Secretin (Porcine) 0 Glucagon (Human) 0 Galanin (Rat) 0 Somatostatin 0 PACAP-38 (Human, Rat, Ovine) 0
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	33.2-434 pg/ml
ED50	96.03 pg/ml
Sample Level	13.5 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.