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Obestatin (Human, Monkey) - Purified IgG Antibody

Catalog# Standard size Price Order
G-031-92 200 µg $495.00

Catalog #G-031-92
Standard Size200 µg
SequencePhe - Asn - Ala - Pro - Phe - Asp - Val - Gly - Ile - Lys - Leu - Ser - Gly - Val - Gln - Tyr - Gln - Gln - His - Ser - Gln - Ala - Leu - NH2
SpeciesHuman
Specific SpecieMonkey
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 120µl of distilled water and 80µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use.
Storage ConditionFor optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
ContentsEach vial contains 200µg of rabbit IgG lyophilized in 120µl PBS (137 mM NaCL, 2.7 mM KCL, 4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH7.5).
Recommended Dilution FactorThis was purified from whole serum by Protein A affinity column. The HPLC purified peptide Obestatin (Mouse, Rat) IgG was detected by this IgG at 1:12800 (0.078ug/ml) on ELISA. We recommend that each laboratory determines an optimum titer for use in its particular assay system.

Applications:
Direct ELISA: 1:3200~6400
Immunohistochemistry: 1:100 ~ 200
Cross ReactivityThis antibody is cross reactivity with obestatin (Human, Monkey) at 100%. It was tested in immunohistochemistry in rat stomach tissue and human placenta tissue.
Image

Protocol:  
 Tissue Sample rat stomach and human placenta tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Obestatin (Human) Purified IgG (Catalog No.:G-031-92)
 Optimal Dilution 1:100~200 (overnight hour at 4oC)
 Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
 Substrate DAB (Sigma), 10"
 Counterstained Hematoxylin, 30 Sec
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