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PYY (3-36)-Gly (Canine, Rat, Mouse, Porcine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-059-09 100 µl $250.00

Image    
Protocol for Immunohistochemistry: 
 Tissue Sample Human Colon
 Fixative 10% formalin
 Embedding Paraffin
 Negative Control No primary antibody
 Pretreatment

 N/A

 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody

 Rabbit Anti-PYY (3-36)-Gly (R,M,C,P) Antiserum (Catalog No.: H-059-09)

 Optimal Dilution

 1:500, 1 hour at RT

 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
 Amplification Streptavidin-HRP (Vector), 1:400, 30 min
 Detection System HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 sec
Catalog #H-059-09
Standard Size100 µl
SpeciesRat, Mouse, Porcine, Canine
HostRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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