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PYY (1-36)-Gly (Rat, Mouse, Porcine, Canine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-059-06 100 µl $250.00

Image

    

Protocol for Immunohistochemistry: 
 Tissue Sample Human Colon
 Fixative 10% formalin
 Embedding Paraffin
 Negative Control No primary antibody
 Pretreatment

 N/A

 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-PYY (1-36)-Gly (H) Antiserum (Catalog No.: H-059-06)
 Optimal Dilution

 1:500, 1 hour at RT

 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
 Amplification Streptavidin-HRP (Vector), 1:400, 30 min
 Detection System HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 sec
Catalog #H-059-06
FamilyGly-extended PYY
Standard Size100 µl
SequenceTyr - Pro - Ala - Lys - Pro - Glu - Ala - Pro - Gly - Glu - Asp - Ala - Ser - Pro - Glu - Glu - Leu - Ser - Arg - Tyr - Tyr - Ala - Ser - Leu - Arg - His - Tyr - Leu - Asn - Leu - Val - Thr - Arg - Gln - Arg - Tyr - Gly
SpeciesRat, Mouse, Porcine, Canine
HostRabbit
ImmunogenGly-extended PYY (1-36) (C,R,M,P)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_G_

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