Neuronostatin-13 (Human, Porcine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human pancreas tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	No
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Neuronostatin-13 (Human, Porcine) Antibody (Catalog No.:H-060-50)
Optimal Dilution	1:200 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-060-50

Family

Neuronostatin

Standard Size

100 ul

Species

Human, Porcine

Host

rabbit

Immunogen

human/porcine Neuronostatin-13

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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Publication that use this product:

Siok L. Dun et al., Neuronostatin is co-expressed with somatostatin and mobilizes calcium in cultured rat hypothalamic neurons.
Neuroscience. 2010 March 17; 166(2): 455–463. doi: 10.1016/j.neuroscience.2009.12.059