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Chemerin / TIG2 (145-157) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-50 100 µl $495.00

Image    

Protocol for Immunohistochemistry:

 Tissue Sample

 Human Hepatoma tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 N/A

 Blocking

 3% H2O2, 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-TIG2 /Chemerin (145-157) (H) Antiserum (Catalog No.: H-002-50)

 Optimal Dilution

 1:500 (1 hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-50
Standard Size100 µl
SpeciesHuman
Specific SpecieRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
Alphabet_C_
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