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Alarin (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-026-33 100 µl $250.00

Image   
Protocol for Immunohistochemistry
 Tissue SampleMouse/Rat Brain Tissue
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Alarin (Rat) Antibody (Catalog No.: H-026-33)

 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-026-33
FamilyAlarin
Standard Size100 µl
SequenceAla - Pro - Ala - His - Arg - Ser - Ser - Thr - Phe - Pro - Gln - Arg - Pro - Thr - Arg - Ala - Gly - Arg - Gln - Thr - Gln - Leu - Leu - Arg - Ser
SpeciesRat
HostRabbit
ImmunogenAlarin (Rat)
Reconstitution100 µl
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry- 1:500
Alphabet_A_

%Alarin%

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