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Catestatin Variant (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-053-28 100 µl $295.00

Image
Protocol for Immunohistochemistry:
 Tissue Sample Human pancreas tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking

 3% H2O2, 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Catestatin (Mouse) Antibody (Cat. No.: H-053-28)

 Optimal Dilution 1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec
Catalog #H-053-28
Standard Size100 µl
SequenceArg - Ser - Met - Arg - Leu - Ser - Phe - Arg - Thr - Arg - Gly - Tyr - Gly - Phe - Arg - Asp - Pro - Gly - Leu - Gln - Leu
SpeciesMouse
HostRabbit
ImmunogenCatestatin (Mouse)
Reconstitution100 ul
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_C_

%053-30%;%053-27%;%053-28%;%053-31%;%053-18%;%053-26%;%053-19%;%053-20%;%053-21%;%053-22%;%053-23%;%053-27%;%053-28%;%053-29%;%053-32%;%053-33%;%053-35%

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