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Spexin, Prepro (36-58) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-023-82 100 µl $450.00

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Protocol for Immunohistochemistry
 Tissue Sample

Human stomach tissues and stomach cancer tissues

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary AntibodyRabbit Anti-Spexin, Prepro (36-58) (H) Antiserum (Catalog No.: H-023-82)
 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-023-82
FamilySpexin
Standard Size100 µl
SequenceAsn - Trp - Thr - Pro - Gln - Ala - Net - Leu - Tyr - Leu - Lys - Gly - Ala - Gln - Gly - Arg - Arg - Phe - Ile - Ser - Asp - Gln - Ser
SpeciesHuman
HostRabbit
ImmunogenSpexin, Prepro (36-58) (Human)
Reconstitution100 ul
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_S_

%023-81%;%023-82%;%023-83%

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