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Adrenomedullin (1-44) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-010-28 100 µl $370.00

Image

    

H-010-28-2444-3-H-500     H-010-28-2444-3-R-500-lung

Protocol for Immunohistochemistry
 Tissue Sample Human/Rat Lung and Lunch Cancer Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No Primary Antibody
 PretreatmentTarget Retrieval 25 min (Steam)
 Blocking2% Normal Goat Serum, 3% H2O2
 Primary AntibodyAnti-Adrenomedullin (1-44) (Human) Antibody (Catalog No.: H-010-28)
 Optimal Dilution1:500 (1hour at RT)
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 AmplificationStreptavidin-HRP (Vector), 1:400, 30 min
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 sec
Catalog #H-010-28
FamilyAdrenomedullin
Standard Size100 µl
SequenceTyr - Arg - Gln - Ser - Met - Asn - Asn - Phe - Gln - Gly - Leu - Arg - Ser - Phe - Gly - Cys - Arg - Phe - Gly - Thr - Cys - Thr - Val - Gln - Lys - Leu - Ala - His - Gln - Ile - Tyr - Gln - Phe - Thr - Asp - Lys - Asp - Lys - Asp - Asn - Val - Ala - Pro - Arg, Disulfide Bridge: Cys16 - Cys21
SpeciesHuman
HostRabbit
ImmunogenAdrenomedullin (1-44) (Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_A_

%010-28%;%010-37%

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